- SouthArc, Inc.
 - Gainesville - Provides archaeological and historical consulting services.
- Biology > Biotechnology
- GenoMechanix
- Alachua - Provides custom oligonucleotides and peptides, gene synthesis, antibodies, and molecular reagents.
- Oragenics
- Alachua - Developing cavity prevention technology and a new broad-spectrum antibiotic.
- Biology > Biotechnology > Regeneration Technologies, Inc.@
- Engineering@
- Instruments
- Highvac Co.
- Inglis - Manufacturer of high vacuum components.
- Software
- Thrall Consulting
- Gainesville - Offers business geography consulting and product services, including advice on GIS installation, site selection, corporate real estate analysis, and custom software development.
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