- African and Middle Eastern Reading Room, Library of Congress
 - Offers African, Hebraic, and Near East sections covering more than 70 counties and regions. - American Folklife Center
- Incorporates the Archive of Folk Culture, which was established at the Library of Congress in 1928, to preserve and present American music, photographs, and films.
- Asian Reading Room
- Includes Chinese, Japanese, Korean, South and Southeast Asia, and Tibetan collections.
- Business Reference Services
- Business division of the Library of Congress reference service. Find information about commerce, statistics, banking, insurance, economics, finance, investment, and marketing, with help from reference librarians.
- European Reading Room
- Primary public access point for readers and researchers seeking to use the vast European collections of the Library of Congress, including those from Russian-speaking areas of Asia.
- Geography and Map Division
- Comprehensive collection of cartographic materials, ranging from ourteenth century portolan charts through recent geographic information systems data sets.
- Hispanic Reading Room
- Includes online collections covering history and genealogy.
- Law Library of Congress, The
- Provides research and legal information to the U.S. Congress and the U.S. Federal Courts and Executive Agencies, and also offers reference services to the public.
- Local History and Genealogy Reading Room, Library of Congress
- One of the world's premier collections of U.S. and foreign genealogical and local historical publications.
- Manuscript Reading Room
- Collection of documents of American history and culture.
- Recorded Sound Reference Center
- Science Reading Room
- Collection covers science, technology, technical reports, and standards.
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