- Biological Resources Division
 - Aims to provide the scientific understanding and technologies needed to support the sound management and conservation of United States biological resources. - Florida Caribbean Science Center
- Conducting research in the southeastern U.S. and Virgin Islands focusing on nonindigenous aquatic species, coral reef ecology, marine studies, and more.
- National Wetlands Research Center
- Biological research related to protecting, restoring, managing national resources, with an emphasis on fish, wildlife, and wetlands in the South.
- National Wildlife Health Center
- Biomedical laboratory dedicated to assessing the impact of disease on wildlife and to identifying the role of various pathogens in contributing to wildlife losses.
- Northern Prairie Wildlife Research Center
- Patuxent Wildlife Research Center (2)
- USGS Fort Collins Science Center
- Conducts ecological research in the interior western United States. Site highlights research projects, scientists, publications, data, and education.
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